Building a Quant Method in SCIEX OS Software for DAD Data

For research use only. Not for use in diagnostic procedures.

Answer

Peaks from DAD or UV chromatograms that were acquired during an LC/MS experiment can be quantified in SCIEX OS software. In a new quantitation method in SCIEX OS software, select the representative sample, and then on the Components tab, select the drop down in the Experiment Index column. Select the DAD experiment for each transition and add the desired wavelength to quantitate in the Start - Stop (Wavelength) column.


Next, change all of the Experiment Index options for each transition to DAD. This can only be done by manually selecting the drop down menu for each analyte. Select the Start Stop column and use the Fill Down option to add the start stop wavelength to each transition.

Remove the Qualifying transitions (2nd and 3rd transitions) for each analyte and add a retention time (RT) value for each analyte. Then select the Integration tab and press Calculate Method Parameters to review the peak integration for each analyte.

At first, the peaks for each analyte will not automatically appear as integrated. To correct this, remove the value from the Minimum peak height field and set it to zero. Select Apply, and peaks should appear as integrated. Repeat this process for each analyte. Save the method, and then build a new results table. The peak area for each DAD peak will be in the Area column.