How to Setup Standard Addition in SCIEX OS Software

For research use only. Not for use in diagnostic procedures.

Answer

Please follow the below steps to enable standard addition columns in a new results table in SCIEX OS software.

1. Open the Analytics workspace.
2. Click Process Method > New.
3. Select the Workflow page and then select at least one workflow and the reference samples.
4. Select Components tab and define the component names, masses, internal standards, groups, etc.
5. Select the Integration page and then select the integration parameters for each component.
6. On the Integration page, select Options > Quantitate by standard addition (see screen shot).

This feature has specific requirements for the following batch fields:
Sample ID: All samples belonging to the same standard addition group must have the same sample ID.
Sample Type: All samples to be quantitated using standard addition must have the sample type, standard.
Actual Concentration: This field must contain the known concentration of standard added to each sample in the standard addition group. For example, for samples with no standard added, it is 0. Data from this column is plotted as the X-axis on the calibration curve.

If this feature is enabled, then the results table contains new columns including Standard Addition Accuracy. Standard Addition Accuracy compares the Standard Addition Calculated Concentration for a sample to the Standard Addition Actual Concentration.