Recommended NIST Library Processing Method Settings


日期: 12/27/2018
类别: SCIEX OS , LibraryView Software , MasterView Software

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For research use only. Not for use in diagnostic procedures.


Answer

The NIST library contains spectra from multiple instrument platforms and spectra that is merged from multiple spectra acquired at various Collision Energy (CE) settings. Therefore, the library search parameters must be carefully optimized to effectively search the library. The figure below shows the recommended library search settings for the MasterView™ 1.1 software and SCIEX OS 1.3.
The recommended library search settings:
• Deactivate the Collision Energy filtering to prevent false negatives.
• Set the Precursor Mass Tolerance to 0.01 Da to accelerate the processing time.
• Increase the Intensity Factor to 10 to account for instrumental differences in relative intensity.
NIST library settings

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