知识库文章

Why does 32 Karat software allow multiple e-signatures across a single data set?

The electronic signature feature in 32 Karat software supports multiple users and allows individual users to sign multiple times.

Setting up a single-injection CID/EAD data-dependent acquisition (DDA) method for the ZenoTOF 7600 system

In SCIEX OS software versions 3.0 and greater, two information-dependent acquisition (IDA) methods can be created in the same acquisition method: one for electron-activated dissociation (EAD) and one for collision-induced dissociation (CID) fragmentation.

Middle-down sMRM HR acquisiton method setup using EAD fragmentation on the ZenoTOF® 7600 system

This article describes the parameters for evaluating subunit proteins using electron-activated dissociation (EAD) fragmentation on the ZenoTOF® 7600 system. Specific values for a successful middle-down, high-resolution sMRM acquisition method using EAD fragmentation are provided. Choosing a low, medium, and high charge state of the protein envelope is critical to get the most diverse fragmentation.

Bio Tool Kit microapp cannot manually sequence peptide MS/MS data from EAD-derived fragments

The Bio Tool Kit microapp embedded in PeakView® or SCIEX OS software cannot perform de novo manual sequencing or MS/MS fragment matching for peptides fragmented using electron activated dissociation (EAD) on a ZenoTOF 7600 system. The MS/MS data obtained by EAD is too complex and fragment-rich, presenting the software with too many options for potential fragment ions; however, the Bio Tool Kit app can process MS/MS data acquired by CID (collision-induced dissociation).

How to use Bio Tool Kit for interpreting peptide MS/MS data acquired with CID and EAD fragmentation?

The Bio Tool Kit and its Peptide Fragments feature is tremendously helpful when trying to interpret MS/MS peptide data. This can be adjusted to either CID (collision-induced dissociation) or EAD (electron activated dissociation). A glycopeptide example for EAD and CID is described.

Processing oligonucleotides using Bio Tool Kit software

The Bio Tool Kit software can deconvolute oligonucleotides in negative mode using the Reconstruct Protein option. The Bio Tool Kit microApp is embedded in the PeakView™ or SCIEX OS software and requires an additional license to activate these features.

Configuring ChemSpider and its databases in SCIEX OS software Explorer mode

To use ChemSpider in the Explorer mode in SCIEX OS software, Formula Finder settings must be configured correctly. After selecting the Show Options icon in Formula Finder, a Formula Finder Settings screen appears. Users must select the ChemSpider Service options in the Dictionaries tab to see the compounds suggested by ChemSpider in the Formula Finder window of the Explorer module. `

How can I process MS1-level oligonucleotides in Molecule Profiler software?

Molecule Profiler software includes workflows for oligonucleotide impurity studies conducted on an MS1-level. To start using these workflows for oligonucleotide studies, launch the software and select Processing Parameters from the Workflow menu on the left panel.

Adding workflow templates to Biologics Explorer software without the template manager

Biological Explorer software uses workflow templates that are typically packaged into the software using a built-in template manager. If the template manager is not working, then templates can be added to the Biologics Explorer software by transferring them to a specific file path on the C drive: C:\ProgramData\SCIEX\BiologicsExplorer\templates.

Restoring and using the script - Manually Integrate - in Analyst® software versions 1.7.2 and 1.7.3

The script - Manually Integrate - is missing in Analyst® software versions 1.7.2 and 1.7.3, although it was previously supported in versions 1.7 and 1.7.1. This articles describes how to restore the script to these later versions of Analyst software. Instructions are provided for integrating peaks of interest with the script that were missed or only partially integrated.