A rapid, robust, sensitive and specific LC-MS/MS assay has been developed for the detection of several major classes of known toxic mycotoxins. The method uses a simple solvent extraction followed by a dilution and injection of extracts to achieve detection of mycotoxins below the regulatory requirements. Fast polarity switching and the Scheduled MRM� algorithm were used with the SCIEX Triple Quad� 5500 system to cover all mycotoxins of interest and to detect them with the best sensitivity, accuracy, and reproducibility.
This seminar will present new LC-MS/MS tools, tips, and workflows designed to help food testing labs overcome these and other every-day obstacles. A preview to future innovations from SCIEX will also be presented.
Multiplexing liquid chromatography (LC) systems, and synchronizing to a single tandem mass spectrometer (MS/MS), can generate the high throughput needed by maximizing the efficiency of the MS/MS detector. An integrated multiplexing system has been specifically designed to synchronize two LC systems and an SCIEX mass spectrometer, allowing injection of samples into two LC streams in parallel. The overlapping LC runs and efficient use of MS detection, achievable with the SCIEX MPX�-2 High Throughput system, is shown to result in an overall higher throughput for common routine analyses.