知识库文章

Bio Tool Kit microapp cannot manually sequence peptide MS/MS data from EAD-derived fragments

The Bio Tool Kit microapp embedded in PeakView® or SCIEX OS software cannot perform de novo manual sequencing or MS/MS fragment matching for peptides fragmented using electron activated dissociation (EAD) on a ZenoTOF 7600 system. The MS/MS data obtained by EAD is too complex and fragment-rich, presenting the software with too many options for potential fragment ions; however, the Bio Tool Kit app can process MS/MS data acquired by CID (collision-induced dissociation).

How to use Bio Tool Kit for interpreting peptide MS/MS data acquired with CID and EAD fragmentation?

The Bio Tool Kit and its Peptide Fragments feature is tremendously helpful when trying to interpret MS/MS peptide data. This can be adjusted to either CID (collision-induced dissociation) or EAD (electron activated dissociation). A glycopeptide example for EAD and CID is described.

Processing oligonucleotides using Bio Tool Kit software

The Bio Tool Kit software can deconvolute oligonucleotides in negative mode using the Reconstruct Protein option. The Bio Tool Kit microApp is embedded in the PeakView™ or SCIEX OS software and requires an additional license to activate these features.