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After taking this training course, you should be able to:

• Understand how to manually determine possible peptide sequences from a proteomics IDA datafile using the ‘Manual Sequence’ function, and link an experimental spectrum to a theoretical peptide sequence;
• Take peptide data and process with the “LC/MS Peptide reconstruct” function to deconvolve spectral peaks and produce a peptide map;
• Utilize the ‘Digest Protein’ function to obtain all theoretical peptide sequences from a protein of interest, and how to link this back to peptide mapping data;
• Use the ‘Manual Reconstruct’ function for both peptide and protein data to identify the MW and potential charge states of a target;
• Obtain a deconvolved, zero charge mass graph for an intact protein target using the ‘Protein Reconstruct’ function

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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